Abstract
Temperature-induced elongation of hypocotyls, petioles, and roots, together with hyponastic leaf responses, constitute key model phenotypes that can be used to assess a plant’s capacity for thermomorphogenesis. Phenotypic responses are often quantified at a single time point during seedling development at different temperatures. However, to capture growth dynamics, several time points need to be assessed, and ideally continuous measurements are taken. Here we describe a general experimental setup and technical solutions for recording and measuring seedling phenotypes at single and multiple time points. Furthermore, we present an R-package called “rootdetectR,” which allows easy processing of hypocotyl, root or petiole length, and growth rate data and provides different options of data presentation.
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Janitza, P., Zhu, Z., Anwer, M.U., van Zanten, M., Delker, C. (2024). A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring. In: Chen, M. (eds) Thermomorphogenesis. Methods in Molecular Biology, vol 2795. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3814-9_1
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DOI: https://doi.org/10.1007/978-1-0716-3814-9_1
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