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Generating Homogeneous Brain Organoids from Human iPSCs

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Cerebral Cortex Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2794))

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Abstract

There is a high demand for the development of in vitro models for human brain development and diseases due to the inaccessibility of human brain tissues. The human iPSC-derived brain organoids provide a promising in vitro model for studying human brain development and disorders. However, it is challenging to generate a large number of brain organoids with high consistency for modeling human neurological diseases. Here, we describe a method for generating high-yield brain organoids with high consistency by combining large-scale embryoid body (EB) generation and incorporating a quality control screening step during differentiation. The method described in this chapter provides a robust way to generate brain organoids for studying human brain development and modeling neurological diseases.

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Acknowledgments

The authors would like to thank Louise and Herbert Horvitz, the Christopher Family, the Judy and Bernard Briskin Fund, and the Sidell Kagan Foundation for their generosity. This work was supported by the National Institute of Aging of the National Institutes of Health under Grant Nos. R01 AG056305, RF1 AG061794, R01 AG072291, and RF1 AG079307 to Y.S.

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Authors and Affiliations

  1. Department of Neurodegenerative Diseases, Beckman Research Institute of City of Hope, Duarte, CA, USA

    Xianwei Chen & Yanhong Shi

Authors
  1. Xianwei Chen
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  2. Yanhong Shi
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Corresponding author

Correspondence to Yanhong Shi .

Editor information

Editors and Affiliations

  1. Institute for Developmental Research, Aichi Developmental Disability Center, Aichi, Japan

    Koh-ichi Nagata

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Chen, X., Shi, Y. (2024). Generating Homogeneous Brain Organoids from Human iPSCs. In: Nagata, Ki. (eds) Cerebral Cortex Development. Methods in Molecular Biology, vol 2794. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3810-1_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3810-1_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3809-5

  • Online ISBN: 978-1-0716-3810-1

  • eBook Packages: Springer Protocols

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