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Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Red Dye

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Characterization of Nanoparticles Intended for Drug Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2789))

Abstract

The induction of oxidative stress by engineered nanomaterials has been associated with cytotoxic and inflammatory responses, damaging healthy cells and tissues. In contrast, when directed against cancer and autoinflammatory diseases, some nanomaterials inducing oxidative stress have also been reported as potential therapies for these disorders. Therefore, studying oxidative stress has become a popular tool not only in toxicology and immunotoxicology but in other areas of biology as well, including those related to developing novel therapies. Total oxidative stress may result from multiple cellular organelles. The protocol described herein allows for the analysis of oxidative stress in mitochondria.

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Acknowledgment

This project has been funded in whole or in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract 75N91019D00024. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Authors and Affiliations

  1. Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA

    Ankit Shah & Marina A. Dobrovolskaia

Authors
  1. Ankit Shah
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  2. Marina A. Dobrovolskaia
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Corresponding author

Correspondence to Marina A. Dobrovolskaia .

Editor information

Editors and Affiliations

  1. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA

    Jeffrey D. Clogston

  2. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA

    Rachael M. Crist

  3. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA

    Marina A. Dobrovolskaia

  4. Nanotechnology Characterization Laboratory Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA

    Stephan T. Stern

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Shah, A., Dobrovolskaia, M.A. (2024). Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Red Dye. In: Clogston, J.D., Crist, R.M., Dobrovolskaia, M.A., Stern, S.T. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 2789. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3786-9_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3786-9_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3785-2

  • Online ISBN: 978-1-0716-3786-9

  • eBook Packages: Springer Protocols

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