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Adult Stem Cells Freezing Processes and Cryopreservation Protocols

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2783))

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Abstract

The development of simple but effective storage protocols for adult stem cells will greatly enhance their use and utility in tissue-engineering applications. Cryopreservation has shown the most promise but is a fairly complex process, necessitating the use of chemicals called cryoprotective agents (CPAs), freezing equipment, and obviously, storage in liquid nitrogen. The purpose of this chapter is to present a general overview of cryopreservation storage techniques and the optimal protocols/results obtained in our laboratory for long-term storage of adult stem cells using freezing storage.

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Acknowledgments

The author thanks Dr. Elizabeth Clubb and Dr. James Wade at the Pennington Biomedical Research Center (PBRC) for supplying the liposuction aspirates and their many patients for consenting to participate in this protocol; Marilyn Dietrick of the LSU School of Veterinary Medicine Flow Cytometry Core Facility; Prof. Jeffrey Gimble, Gang Yu, Xiying Wu, of the Stem Cell Biology Laboratory at the Pennington Biomedical Research Center (PBRC), and the clinical nutrition research unit (CNRU) Molecular Mechanism Core at PBRC for their technical assistance. Acknowledgments are also due to Dr. Gimble, a longtime colleague and without his help this work would never have been initiated. In addition, acknowledgments are also due to Dr. S. Thirumala (freezing experiments), R. Fuller (comparing the freezing devices, CRF and DSS), Dr. S. Shaik (HSP experiments) results reported in this chapter. This work was supported in part by funding from the Louisiana Board of Regents, the Department of Mechanical Engineering at the Louisiana State University, the institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health (NIH) under award number R21DK91852 and by the institute of General Medical Sciences of the NIH under award number R15GM141653.

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Authors and Affiliations

  1. Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA

    Mohan Kumar Dey & Ram V. Devireddy

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  1. Mohan Kumar Dey
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  2. Ram V. Devireddy
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Correspondence to Ram V. Devireddy .

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Editors and Affiliations

  1. Obatala Sciences Inc, New Orleans, LA, USA

    Jeffrey Gimble

  2. Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, TX, USA

    Bruce Bunnell

  3. Obatala Sciences Inc, New Orleans, LA, USA

    Trivia Frazier

  4. Obatala Sciences Inc, New Orleans, LA, USA

    Cecilia Sanchez

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Dey, M.K., Devireddy, R.V. (2024). Adult Stem Cells Freezing Processes and Cryopreservation Protocols. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_5

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  • DOI: https://doi.org/10.1007/978-1-0716-3762-3_5

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