Abstract
Microglia and oligodendrocyte precursor cells (OPCs) are critical glia subsets in the central nervous system (CNS) and are actively engaged in a body of diseases, such as stroke, Alzheimer’s disease, multiple sclerosis, etc. Microglia and OPC serve as compelling tools for the study of CNS diseases as well as the repair and damage of myelin sheath in vitro. In this protocol, we summarized a method which is capable of using the same batch of new-born mice to isolate and culture microglia and OPCs. It integrates the characteristics of practicality, convenience, and efficiency, providing a convenient, easy, and reliable technique for research.
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Acknowledgement
This study was supported by the National Natural Science Fund for Excellent Young Scholars (82022019 to C.-J. Zhang), Nanjing Special Fund for Medical Science and Technology Development Projects for Distinguished Young Scholars (JQX19005 to C.-J. Zhang), and National Natural Science Foundation of China (81701235 to C.-J. Zhang, 81991514 to C.-J. Zhang).
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Li, Z., Zhang, CJ. (2024). Isolation and Culture of Mouse Primary Microglia and Oligodendrocyte Precursor Cells. In: Kumar, V. (eds) Immune Homeostasis. Methods in Molecular Biology, vol 2782. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3754-8_13
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DOI: https://doi.org/10.1007/978-1-0716-3754-8_13
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