Abstract
To examine the mucin-utilizing capacity of bacterial isolates from fecal samples, an in vitro cultivation method using mucins as a carbon source should be considered. This chapter describes a practical method for cultivating bacteria in media containing mucin glycoproteins; for this cultivation method, several factors are considered due to the physical nature of mucin glycoproteins.
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Acknowledgements
This work was partly supported by a grant-in-aid from the JSPS Research Fellowship (21J15883 to HT), the Institution for Fermentation, Osaka (to ToK), and the Kyoto University Foundation (2022 to ToK).
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Takada, H., Katayama, T., Katoh, T. (2024). Cultivation of Microorganisms in Media Supplemented with Mucin Glycoproteins. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_27
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DOI: https://doi.org/10.1007/978-1-0716-3670-1_27
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3669-5
Online ISBN: 978-1-0716-3670-1
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