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Production of Influenza Virus Glycoproteins Using Insect Cells

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Recombinant Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2762))

Abstract

The baculovirus/insect cell expression system is a very useful tool for reagent and antigen generation in vaccinology, virology, and immunology. It allows for the production of recombinant glycoproteins, which are used as antigens in vaccination studies and as reagents in immunological assays. Here, we describe the process of recombinant glycoprotein production using the baculovirus/insect cell expression system.

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Acknowledgments

The Krammer laboratory receives support for work in the immunology, virology, therapeutics, and vaccine space from the NIAID Centers of Excellence for Influenza Research and Response (CEIRR, 75N93021C00014) and Collaborative Influenza Vaccine Innovation Centers (CIVICs, 75N93019C00051) contracts as well as NIAID grants and contracts R01 AI146101, U19 AI168631, R01 AI154470, U19 AI162130, R01 AI137146, U01 AI144616, HHSN272201800048C and U19 AI118610. Additional support comes from FluLab and the Bill and Melinda Gates Foundation. The laboratory is also supported in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under U54 CA260560 and under Contract number 75N91021F00001 via 21X092F1 Mod 01. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

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Authors and Affiliations

  1. Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Madhumathi Loganathan, Benjamin Francis & Florian Krammer

  2. Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Madhumathi Loganathan, Benjamin Francis & Florian Krammer

  3. Department of Pathology, Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Florian Krammer

Authors
  1. Madhumathi Loganathan
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  2. Benjamin Francis
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  3. Florian Krammer
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Corresponding author

Correspondence to Florian Krammer .

Editor information

Editors and Affiliations

  1. Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, USA

    Steven B. Bradfute

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Loganathan, M., Francis, B., Krammer, F. (2024). Production of Influenza Virus Glycoproteins Using Insect Cells. In: Bradfute, S.B. (eds) Recombinant Glycoproteins. Methods in Molecular Biology, vol 2762. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3666-4_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3666-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3665-7

  • Online ISBN: 978-1-0716-3666-4

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