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Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell

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Single Cell Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2752))

Abstract

Many biological or pathological processes are driven by cells difficult to identify or isolate, i.e., rare cells. Very often, these cells have elusive biology. Therefore, their detailed characterization is of utmost importance. There are many approaches that allow analysis of few or even many targets within one class of biomacromolecules/analytes (e.g., DNA, RNA, proteins, etc.) in single cells. However, due to rarity of the cells of interest, there is a great need to comprehensively analyze multiple analytes within these cells, in other words to perform multi-omics analysis. In this chapter, I describe a method to isolate, separate, and amplify total mRNA and genomic DNA of a single cells, using whole transcriptome (WTA) and whole genome amplification (WGA). These WTA and WGA products enable simultaneous analysis of transcriptome and genome of a single cell using various downstream high-throughput approaches.

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Authors and Affiliations

  1. Department of Urology, University Hospital Regensburg, Regensburg, Germany

    Miodrag Gužvić

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  1. Miodrag Gužvić
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Correspondence to Miodrag Gužvić .

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  1. Department of Urology, University Hospital Regensburg, Regensburg, Germany

    Miodrag Gužvić

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Gužvić, M. (2024). Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell. In: Gužvić, M. (eds) Single Cell Analysis. Methods in Molecular Biology, vol 2752. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3621-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3621-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3620-6

  • Online ISBN: 978-1-0716-3621-3

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