Abstract
Ciliated epithelia are common in various human organs, indeed across many species, and their physiological functions are vital. A number of diseases, of genetic, degenerative, or infectious nature, compromise motile cilia function and lead to severe downstream consequences. Culture of ciliated tissues is a common research approach. We focus here on the video microscopy and analysis pipelines developed over the last few years to phenotype ciliary beating in lung cells, specifically to extract: cilia coverage; ciliary beat frequency distributions; the scale for ciliary dynamical coordination; and cilia beat waveform.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Ehrhardt C, Forbes M, Kim KJ (2008) In vitro models of the tracheo-bronchial epithelium. In: Ehrhardt C, Kim KJ (eds) Drug absorption studies, Biotechnology: pharmaceutical aspects, vol VII. Springer, Boston
Sone N, Konishi S, Igura K et al (2021) Multicellular modeling of ciliopathy by combining iPS cells and microfluidic airway-on-a-chip technology. Sci Transl Med 13(601):eabb1298
Berical A, Lee RE, Lu J et al (2022) A multimodal iPSC platform for cystic fibrosis drug testing. Nat Commun 13:4270
Bustamante-Marin XM, Ostrowski LE (2017) Cilia and mucociliary clearance. Cold Spring Harb Perspect Biol 9(4):a028241
Cicuta P (2020) The use of biophysical approaches to understand ciliary beating. Biochem Soc Trans 48(1):221–229
Ehre C, Ridley C, Thornton DJ (2014) Cystic fibrosis: an inherited disease affecting mucin-producing organs. Int J Biochem Cell Biol 52:136–145
Boucher RC (2007) Airway surface dehydration in cystic fibrosis: pathogenesis and therapy. Annu Rev Med 58:157–170
Chilvers MA, Rutman A, O’Callaghan C (2003) Ciliary beat pattern is associated with specific ultrastructural defects in primary ciliary dyskinesia. J Allergy Clin Immunol 112(3):518–524
Legendre M, Zaragosi LE, Mitchison HM (2021) Motile cilia and airway disease. Semin Cell Dev Biol 110:19–33
Tilley AE, Walters MS, Shaykhiev R, Crystal RG (2015) Cilia dysfunction in lung disease. Annu Rev Physiol 77:379–406
Houtmeyers E, Gosselink R, Gayan-Ramirez G, Decramer M (1999) Regulation of mucociliary clearance in health and disease. Eur Respir J 13(5):1177–1188
Kirch J, Guenther M, Doshi N et al (2012) Mucociliary clearance of micro- and nanoparticles is independent of size, shape and charge – an ex vivo and in silico approach. J Control Release 159(1):128–134
Rogers T, Ostrowski L, Livraghi-Butrico A et al (2018) Mucociliary clearance in mice measured by tracking trans-tracheal fluorescence of nasally aerosolized beads. Sci Rep 8:14744
Fradique R, Causa E, Delahousse C, Kotar J, Pinte L, Vallier L, Vila-Gonzalez M, Cicuta P (2023) Assessing motile cilia coverage and beat frequency in mammalian in-vitro cell culture tissues. bioRxiv. https://doi.org/10.1101/2023.03.22.533861
Feriani L, Juenet M, Fowler CJ et al (2017) Assessing the collective dynamics of motile cilia in cultures of human airway cells by multiscale DDM. Biophys J 113(1):109–119
Chioccioli M, Feriani L, Nguyen Q et al (2019) Quantitative high-speed video profiling discriminates between DNAH11 and HYDIN variants of primary ciliary dyskinesia. Am J Respir Crit Care Med 199(11):1436–1438
Chioccioli M, Feriani L, Nguyen Q et al (2019) A how-to guide to: Quantitative high-speed video profiling to discriminate between variants of primary ciliary dyskinesia. bioRxiv. https://doi.org/10.1101/614966
Fulcher ML, Randell SH (2013) Human nasal and tracheo-bronchial respiratory epithelial cell culture. Methods Mol Biol 945:109–121
Chioccioli M, Feriani L, Kotar J, Bratcher PE, Cicuta P (2019) Phenotyping ciliary dynamics and coordination in response to CFTR-modulators in cystic fibrosis respiratory epithelial cells. Nat Commun 10(1):1763
Cerbino R, Trappe V (2008) Differential dynamic microscopy: probing wave vector dependent dynamics with a microscope. Phys Rev Lett 100(18):188102
Acknowledgments
Funding for this work was from the Cystic Fibrosis Trust grant SRC016 and the EU EC MSCA-ITN grant PhyMot. We particularly thank our former co-workers who developed much of the tools described here: L. Feriani, N. Pellicciotta, and M.Chioccioli.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Causa, E., Fradique, R., Cicuta, P. (2024). Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods. In: Mennella, V. (eds) Cilia. Methods in Molecular Biology, vol 2725. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3507-0_16
Download citation
DOI: https://doi.org/10.1007/978-1-0716-3507-0_16
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3506-3
Online ISBN: 978-1-0716-3507-0
eBook Packages: Springer Protocols