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Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue

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Plant-Virus Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2724))

Abstract

Reverse transcription quantitative PCR (RT-qPCR) allows sensitive and specific measurement of mRNA transcripts from a given sample in a short period of time. Relative and absolute RT-qPCR are two strategies that could be used to quantify mRNA transcripts, based on the goal of the experiment. Here, we describe the protocol for the quantification of plant viral RNA transcripts from an infected sample using both strategies.

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Acknowledgements

We thank the Academy of Finland (Grant 1332950) for KM and Doctoral Programme in Plant Sciences, University of Helsinki, for PD for financial support.

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Authors and Affiliations

  1. Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

    Pinky Dutta & Kristiina Mäkinen

Authors
  1. Pinky Dutta
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  2. Kristiina Mäkinen
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Corresponding author

Correspondence to Kristiina Mäkinen .

Editor information

Editors and Affiliations

  1. Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

    Elizabeth P.B. Fontes

  2. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Kristiina Mäkinen

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Dutta, P., Mäkinen, K. (2024). Absolute and Relative Quantification of Single-Stranded Positive-Sense RNA Viruses from Plant Tissue. In: Fontes, E.P., Mäkinen, K. (eds) Plant-Virus Interactions. Methods in Molecular Biology, vol 2724. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3485-1_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3485-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3484-4

  • Online ISBN: 978-1-0716-3485-1

  • eBook Packages: Springer Protocols

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