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Generation of Organoids and Analysis of Ferroptosis in Organoids

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Ferroptosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2712))

Abstract

Ferroptosis is a unique form of iron-dependent cell death induced by lipid peroxidation and subsequent plasma membrane rupture, which sets it apart from other types of regulated cell death. Ferroptosis has been linked to a diverse range of biological processes, such as aging, immunity, and cancer. Organoids, on the other hand, are three-dimensional (3D) miniaturized model systems of different organs in vitro cultures, which have gained widespread interest for modeling tissue development and disease, drug screening, and cell therapy. Organoids offer tremendous potential for improving our understanding of human diseases, particularly in the search for the field of ferroptosis in pathological processes of organs. Furthermore, cancer organoids are utilized to investigate molecular mechanisms and drug screening in vitro due to the anti-tumor effect of ferroptosis. Currently, the development of liver organoids has reached a relatively mature stage. Here, we present the protocols for the generation of liver organoids and liver cancer organoids, along with the methods for detecting ferroptosis in organoids.

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Author information

Author notes

  1. Authors Wenxin Li and Yujie Su have equally contributed to this chapter.

Authors and Affiliations

  1. CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, China

    Wenxin Li, Yujie Su, Jingyi Guo, Mengfei Wang & Xingguo Liu

  2. Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, China-New Zealand Joint Laboratory on Biomedicine and Health, CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Institute for Stem Cell and Regeneration, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China

    Wenxin Li, Yujie Su, Mengfei Wang & Xingguo Liu

  3. University of Chinese Academy of Sciences, Beijing, China

    Wenxin Li, Yujie Su & Mengfei Wang

  4. Innovation Centre for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

    Jingyi Guo

  5. Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong, SAR, China

    Xingguo Liu

Authors
  1. Wenxin Li
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  2. Yujie Su
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  3. Jingyi Guo
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  4. Mengfei Wang
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  5. Xingguo Liu
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Corresponding author

Correspondence to Xingguo Liu .

Editor information

Editors and Affiliations

  1. Centre de Recherche des Cordeliers, Université de Paris, Paris, France

    Guido Kroemer

  2. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Daolin Tang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Li, W., Su, Y., Guo, J., Wang, M., Liu, X. (2023). Generation of Organoids and Analysis of Ferroptosis in Organoids. In: Kroemer, G., Tang, D. (eds) Ferroptosis. Methods in Molecular Biology, vol 2712. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3433-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3433-2_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3432-5

  • Online ISBN: 978-1-0716-3433-2

  • eBook Packages: Springer Protocols

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