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Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation

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Inflammation and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2691))

Abstract

Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8+ T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs.

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Author notes

  1. Julian P. Vivian and Pouya Faridi contributed equally for this chapter.

Authors and Affiliations

  1. Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, VIC, Australia

    Dongbin Jin, Tima Shamekhi, Terry C. C. Lim Kam Sian & Pouya Faridi

  2. Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia

    Khai Lee Loh, Yi Tian Ting & Joshua D. Ooi

  3. Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

    Terry C. C. Lim Kam Sian & Pouya Faridi

  4. St Vincent’s Institute of Medical Research, Fitzroy, VIC, Australia

    James Roest & Julian P. Vivian

  5. Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia

    Julian P. Vivian

Authors
  1. Dongbin Jin
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  2. Khai Lee Loh
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  3. Tima Shamekhi
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  4. Yi Tian Ting
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  5. Terry C. C. Lim Kam Sian
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  6. James Roest
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  7. Joshua D. Ooi
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  8. Julian P. Vivian
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  9. Pouya Faridi
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Corresponding author

Correspondence to Julian P. Vivian .

Editor information

Editors and Affiliations

  1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, VIC, Australia

    Brendan J. Jenkins

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Jin, D. et al. (2023). Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation. In: Jenkins, B.J. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 2691. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3331-1_25

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  • DOI: https://doi.org/10.1007/978-1-0716-3331-1_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3330-4

  • Online ISBN: 978-1-0716-3331-1

  • eBook Packages: Springer Protocols

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