Abstract
Histology is a technique used to study the morphology of cell structures by cutting samples into thin sections. Histological cross-section and staining are the techniques needed to visualize the morphology of cell tissues. A suitable tissue staining experiment was created to observe changes in the retinal layer in zebrafish embryos. Zebrafish have a human-like visual system, retina, and eye structures. Due to the small size of zebrafish and undeveloped bones in the embryonic stage, the resistance through cross-section is inevitably small. Here, we present optimized protocol changes in eye tissue of zebrafish using frozen blocks.
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This work was supported by EYEBIOKOREA Inc. and Inje University Busan Paik Hospital.
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Lee, Y., Yang, J. (2023). Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo. In: Liu, GS., Wang, JH. (eds) Diabetic Retinopathy. Methods in Molecular Biology, vol 2678. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3255-0_15
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DOI: https://doi.org/10.1007/978-1-0716-3255-0_15
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3254-3
Online ISBN: 978-1-0716-3255-0
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