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Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline

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Diabetic Retinopathy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2678))

Abstract

Retinal diseases like diabetic retinopathy and age-related macular degeneration affect millions of individuals worldwide and often lead to vision loss. Vitreous fluid abuts the retina, is accessible for sampling, and contains many proteins related to retinal disease. Therefore, analysis of vitreous is an important tool for studying retinal disease. Because it is rich in proteins and extracellular vesicles, mass spectrometry-based proteomics is an excellent method for vitreous analysis. Here, we discuss important variables to consider when performing vitreous proteomics via mass spectrometry.

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Author information

Authors and Affiliations

  1. Department of Ophthalmology, Penn State College of Medicine, Hershey, PA, USA

    Sarah Weber, Yuanjun Zhao & Jeffrey Sundstrom

  2. Bioinformatics Core, Biomedical Research Core Facilities, University of Michigan Medical School, Ann Arbor, MI, USA

    Nick Carruthers & Chris Gates

Authors
  1. Sarah Weber
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  2. Nick Carruthers
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  3. Chris Gates
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  4. Yuanjun Zhao
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  5. Jeffrey Sundstrom
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Corresponding author

Correspondence to Jeffrey Sundstrom .

Editor information

Editors and Affiliations

  1. Centre for Eye Research Australia, University of Melbourne, East Melbourne, VIC, Australia

    Guei-Sheung Liu

  2. Centre for Eye Research Australia, University of Melbourne, East Melbourne, VIC, Australia

    Jiang-Hui Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Weber, S., Carruthers, N., Gates, C., Zhao, Y., Sundstrom, J. (2023). Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline. In: Liu, GS., Wang, JH. (eds) Diabetic Retinopathy. Methods in Molecular Biology, vol 2678. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3255-0_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3255-0_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3254-3

  • Online ISBN: 978-1-0716-3255-0

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