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Epitope Binning of Monoclonal and Polyclonal Antibodies by Biolayer Interferometry

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Computational Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2673))

Abstract

Understanding the epitopes of antibodies elicited by infection and vaccination is often useful in immunogen design. In this chapter, we describe biolayer interferometry (BLI)-based methods to evaluate such epitopes and permit simultaneous analysis of antibodies from several sources, including monoclonal antibodies (mAbs) and polyclonal serum antibodies (pAbs). Using previously characterized antibodies with known epitopes as controls, the distribution of epitopes for the influenza hemagglutinin (HA) is shown for isolated human mAbs and pooled serum from HA-immunized mice. This method is versatile, high-throughput, and can be adapted to several antigens.

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Acknowledgments

This work was funded by 1R01AI143865 (JJM) and 1K01OD026569 (JJM) and by the Collaborative Influenza Vaccine Innovation Centers (CIVIC) contract by the National Institute of Allergy and Infectious Diseases, a component of the NIH, Department of Health and Human Services, under contract 75N93019C00052.

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Authors and Affiliations

  1. Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA

    Kaito Nagashima & Jarrod J. Mousa

  2. Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA

    Kaito Nagashima & Jarrod J. Mousa

  3. Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences, University of Georgia, Athens, GA, USA

    Jarrod J. Mousa

Authors
  1. Kaito Nagashima
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  2. Jarrod J. Mousa
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Corresponding author

Correspondence to Jarrod J. Mousa .

Editor information

Editors and Affiliations

  1. School of Medicine, Department of Immunology, Complutense University of Madrid, Madrid, Spain

    Pedro A. Reche

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Nagashima, K., Mousa, J.J. (2023). Epitope Binning of Monoclonal and Polyclonal Antibodies by Biolayer Interferometry. In: Reche, P.A. (eds) Computational Vaccine Design. Methods in Molecular Biology, vol 2673. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3239-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3239-0_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3238-3

  • Online ISBN: 978-1-0716-3239-0

  • eBook Packages: Springer Protocols

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