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Fluorescent Fusion Protein Expression in Plant Cells

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Advanced Methods in Structural Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2652))

Abstract

Fluorescent proteins (FPs) revolutionized the cell biology research by visualizing the dynamics of cellular events. In fusion with the targeted proteins, the FPs can be utilized to monitor the protein dynamics and localization in cells. Recently, FPs have been used as reporters for live cell imaging to study the protein localization or organelles dynamics in plants, allowing cell biologists to explore the plant cell function by obtaining tremendous details of cell structures and functions in combination with confocal imaging. To facilitate the usage of fluorescent proteins for protein localization and dynamic analysis in plant cell biology research, here we describe the updated protocol of Agrobacterium-mediated transformation of Arabidopsis thaliana using fluorescent proteins to generate the stable expression transgenic plants for protein trafficking and localization study. We further use the GFP-tagged SDP1 (sugar-dependent protein) lipase, mCherry-tagged peroxisome marker, and BODYPY or Nile Red (lipid droplet staining dye) as examples to introduce the method for the protein localization analysis in plants.

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Acknowledgments

We thank all the members in the lab for developing this protocol.

Author Contributions

S.H. and Y.Z. designed the concept and the organization of the manuscript; S.H. wrote the manuscript; Y.Z. edited the manuscript.

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Authors and Affiliations

  1. The South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China

    Shuxian Huang & Zeng Yonglun

Authors
  1. Shuxian Huang
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  2. Zeng Yonglun
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Editor information

Editors and Affiliations

  1. Universidade da Beira Interior, CICS-UBI, Covilhã, Portugal

    Ângela Sousa

  2. University of Beira Interior, Covilhã, Portugal

    Luis Passarinha

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Huang, S., Yonglun, Z. (2023). Fluorescent Fusion Protein Expression in Plant Cells. In: Sousa, Â., Passarinha, L. (eds) Advanced Methods in Structural Biology. Methods in Molecular Biology, vol 2652. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3147-8_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3147-8_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3146-1

  • Online ISBN: 978-1-0716-3147-8

  • eBook Packages: Springer Protocols

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