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Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo

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Bacterial and Archaeal Motility

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2646))

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Abstract

The bacterial flagellum employs a rotary motor embedded on the cell surface. The motor consists of the stator and rotor elements and is driven by ion influx (typically H+ or Na+) through an ion channel of the stator. Ion influx induces conformational changes in the stator, followed by changes in the interactions between the stator and rotor. The driving force to rotate the flagellum is thought to be generated by changing the stator-rotor interactions. In this chapter, we describe two methods for investigating the interactions between the stator and rotor: site-directed in vivo photo-crosslinking and site-directed in vivo cysteine disulfide crosslinking.

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Acknowledgments

This work was supported in part by JSPS KAKENHI grant numbers 18K07108 and 21K07022 (to H.T.), 18K19293 (to S.K.), and 20H03220 (to M.H.).

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Authors and Affiliations

  1. Department of Bacteriology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan

    Hiroyuki Terashima

  2. Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan

    Michio Homma & Seiji Kojima

Authors
  1. Hiroyuki Terashima
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  2. Michio Homma
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  3. Seiji Kojima
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Corresponding author

Correspondence to Hiroyuki Terashima .

Editor information

Editors and Affiliations

  1. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan

    Tohru Minamino

  2. Graduate School of Science, Osaka Metropolitan University, Osaka, Japan

    Makoto Miyata

  3. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan

    Keiichi Namba

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Terashima, H., Homma, M., Kojima, S. (2023). Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo. In: Minamino, T., Miyata, M., Namba, K. (eds) Bacterial and Archaeal Motility. Methods in Molecular Biology, vol 2646. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3060-0_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3060-0_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3059-4

  • Online ISBN: 978-1-0716-3060-0

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