Abstract
Fluorescence-activated cell sorting (FACS) is a powerful and requisite tool for the analysis and purification of adult stem cells. However, it is difficult to separate adult stem cells from solid organs than from immune-related tissues/organs. This is because of the presence of large amounts of debris, which increases noise in the FACS profiles. In particular, it is extremely difficult for unfamiliar researchers to identify muscle stem cell (also known as muscle satellite cell: MuSC) fraction because all myofibers, which are mainly composed of skeletal muscle tissues, become debris during cell preparation. This chapter describes our FACS protocol, which we have used for more than a decade, to identify and purify MuSCs.
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Acknowledgments
We acknowledge the funding support of the Japan Society for the Promotion of Science (a Grant-in-Aid for Scientific Research (B), 19H04000). M.K. received funding from the Fuji Seal Foundation.
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Kubota, M., Zhang, L., Fukada, Si. (2023). Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells. In: Asakura, A. (eds) Skeletal Muscle Stem Cells. Methods in Molecular Biology, vol 2640. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3036-5_1
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DOI: https://doi.org/10.1007/978-1-0716-3036-5_1
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3035-8
Online ISBN: 978-1-0716-3036-5
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