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Transgenesis and Genome Engineering: A Historical Review

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Transgenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2631))

Abstract

Our ability to modify DNA molecules and to introduce them into mammalian cells or embryos almost appears in parallel, starting from the 1970s of the last century. Genetic engineering techniques rapidly developed between 1970 and 1980. In contrast, robust procedures to microinject or introduce DNA constructs into individuals did not take off until 1980 and evolved during the following two decades. For some years, it was only possible to add transgenes, de novo, of different formats, including artificial chromosomes, in a variety of vertebrate species or to introduce specific mutations essentially in mice, thanks to the gene-targeting methods by homologous recombination approaches using mouse embryonic stem (ES) cells. Eventually, genome-editing tools brought the possibility to add or inactivate DNA sequences, at specific sites, at will, irrespective of the animal species involved. Together with a variety of additional techniques, this chapter will summarize the milestones in the transgenesis and genome engineering fields from the 1970s to date.

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Acknowledgments

This work was supported by the Spanish Ministry of Science and Innovation (MICINN) through the Grant [RTI2018-101223-B-I00] to L.M.

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Authors and Affiliations

  1. National Centre for Biotechnology (CNB-CSIC) and Center for Biomedical Network Research on Rare Diseases (CIBERER-ISCIII), Madrid, Spain

    Lluis Montoliu

  2. National Centre for Biotechnology (CNB-CSIC), Madrid, Spain

    Lluis Montoliu

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  1. Lluis Montoliu
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Correspondence to Lluis Montoliu .

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  1. Transgenic Animal Model Core, University of Michigan, Ann Arbor, MI, USA

    Thomas L. Saunders

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Montoliu, L. (2023). Transgenesis and Genome Engineering: A Historical Review. In: Saunders, T.L. (eds) Transgenesis. Methods in Molecular Biology, vol 2631. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2990-1_1

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