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Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion

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Serum/Plasma Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2628))

Abstract

Platelets are specialized cellular elements of blood and play a central role in maintaining normal hemostasis, wound healing, and host defense but also are implicated in pathologic processes of thrombosis, inflammation, and tumor progression and dissemination. Transfusion of platelet concentrates is an important treatment for thrombocytopenia (low platelet count) due to disease or significant blood loss, with the goal being to prevent bleeding or to arrest active bleeding. In blood circulation, platelets are in a resting state; however, when triggered by a stimulus, such as blood vessel injury, become activated (also termed procoagulant). Platelet activation is the basis of their biological function to arrest active bleeding, comprising a complex interplay of morphological phenotype/shape change, adhesion, expression of signaling molecules, and release of bioactive factors, including extracellular vesicles/microparticles. Advances in high-throughput mRNA and protein profiling techniques have brought new understanding of platelet biological functions, including identification of novel platelet proteins and secreted molecules, analysis of functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. However, because platelets are very easily activated, it is important to understand the different in vitro methods for platelet isolation commonly used and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from human whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.

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Author information

Authors and Affiliations

  1. Transfusion Science, Melbourne, VIC, Australia

    Rosemary L. Sparrow

  2. School of Public Health and Preventive Medicine, Monash University, Melbourne, VIC, Australia

    Rosemary L. Sparrow

  3. Department of Biochemistry and Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, VIC, Australia

    Richard J. Simpson

  4. Baker Heart and Diabetes Institute, Melbourne, VIC, Australia

    David W. Greening

  5. Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University, Melbourne, VIC, Australia

    David W. Greening

  6. Central Clinical School, Monash University, Melbourne, VIC, Australia

    David W. Greening

  7. Baker Department of Cardiometabolic Health, University of Melbourne, Melbourne, VIC, Australia

    David W. Greening

Authors
  1. Rosemary L. Sparrow
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  2. Richard J. Simpson
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  3. David W. Greening
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Corresponding authors

Correspondence to Rosemary L. Sparrow or David W. Greening .

Editor information

Editors and Affiliations

  1. Molecular Proteomics, Baker Heart and Diabetes Institute, Melbourne, VIC, Australia

    David W. Greening

  2. Department of Biochemistry and Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe Institute for Molecular Science, Melbourne, VIC, Australia

    Richard J. Simpson

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sparrow, R.L., Simpson, R.J., Greening, D.W. (2023). Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion. In: Greening, D.W., Simpson, R.J. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 2628. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2978-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2978-9_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2977-2

  • Online ISBN: 978-1-0716-2978-9

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