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Clinical Applications of Nucleic Acid Amplification

  • Book
  • © 2023

Overview

Editors:
  1. Meagan B. Myers

    1. Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Cynthia A. Schandl

    1. Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2621)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Foundations for Successful Assays

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Brief Practical Guide to PCR

      • Page B. McKinzie, Meagan B. Myers
      Pages 3-13

    3. Managing a PCR Contamination Event in a Molecular Pathology Laboratory

      • Julie Woolworth Hirschhorn, Alexandria Avery, Cynthia A. Schandl
      Pages 15-26

    4. Next-Generation Sequencing Informatic Architecture Considerations

      • W. Bailey Glen, Cynthia A. Schandl
      Pages 27-37

  3. Applications in Oncology

    1. Front Matter

      Pages 39-39
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    2. Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants

      • Ieva Keraite, Virginia Alvarez-Garcia, Nicholas R. Leslie
      Pages 41-56

    3. MYD88 L265P Mutation Detection by ddPCR: Recommendations for Screening and Minimal Residual Disease Monitoring

      • Daniela Drandi, Martina Ferrante, Michela Borriero, Simone Ferrero
      Pages 57-72

    4. Capture Methylation-Sensitive Restriction Enzyme Sequencing (Capture MRE-Seq) for Methylation Analysis of Highly Degraded DNA Samples

      • Xiaoyun Xing, Jennifer A. Karlow, Daofeng Li, Hyo Sik Jang, Hyung Joo Lee, Ting Wang
      Pages 73-89

    5. LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genotyping and Microsatellite Instability Detection

      • Sophie I. Jeanjean, Victor Renault, Antoine Daunay, Yimin Shen, Lise M. Hardy, Jean-François Deleuze et al.
      Pages 91-109

    6. Liquid Biopsy for Advanced Cancer: An Amplicon-Based Massively Parallel Sequencing Panel Approach to Precision Oncology

      • Kristen Maurer, Cynthia A. Schandl
      Pages 111-126

    7. Mate Pair Sequencing: Next-Generation Sequencing for Structural Variant Detection

      • Beth A. Pitel, Eric Zimmerman Zuckerman, Linda B. Baughn
      Pages 127-149

  4. Applications in the Management of Organ and Stem Cell Transplantation

    1. Front Matter

      Pages 151-151
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    2. Cell Subset-Specific Chimerism Testing by Short Tandem Repeats Analysis for Engraftment Monitoring After Hematopoietic Stem Cell Transplantation

      • Louise Pagaduan, Thea dela Cruz, Lain Tan, Cassie Wu, Jack Lai, Young Cho et al.
      Pages 153-186

    3. Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation

      • Cassie Wu, Thea dela Cruz, Jack Lai, Denice Kong, Raja Rajalingam
      Pages 187-213

  5. Applications in Constitutional Genetics and Genomics

    1. Front Matter

      Pages 215-215
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    2. Rapid Whole Genome Sequencing for Diagnosis of Single Locus Genetic Diseases in Critically Ill Children

      • Mallory J. Owen, Sergey Batalov, Katarzyna A. Ellsworth, Meredith Wright, Sylvia Breeding, Kwon Hugh et al.
      Pages 217-239

    3. EOSAL–CNV for Easy and Rapid Detection of CNVs by Fragment Analysis

      • Francisco Lara-Hernandez, Jessica Cortez, Soraya Garcia-Sorribes, Sebastian Blesa, M. Dolores Olivares, Andy S. Alic et al.
      Pages 241-253

  6. Applications in Epidemiology and Public Health

    1. Front Matter

      Pages 255-255
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    2. Rapid Cycle and Extreme Polymerase Chain Reaction

      • Carl T. Wittwer
      Pages 257-266
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Keywords

About this book

This volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Clinical Applications of Nucleic Acid Amplification is a valuable resource for both novice and expert scientists in this developing field.

Editors and Affiliations

  • Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, USA

    Meagan B. Myers

  • Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, USA

    Cynthia A. Schandl

Bibliographic Information

  • Book Title: Clinical Applications of Nucleic Acid Amplification

  • Editors: Meagan B. Myers, Cynthia A. Schandl

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2950-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2949-9Published: 12 April 2023

  • Softcover ISBN: 978-1-0716-2952-9Due: 29 July 2023

  • eBook ISBN: 978-1-0716-2950-5Published: 11 April 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 345

  • Number of Illustrations: 17 b/w illustrations, 67 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biomaterials, Biochemistry, general

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