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Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization of Chondroitin Sulfate Proteoglycans

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Proteoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2619))

Abstract

Chondroitin sulfate proteoglycans (CSPGs) are polyanionic extra/pericellular matrix macromolecules that surround almost all cell types and create microenvironmental niches to support miscellaneous cellular events. In general, the multifunctional properties of CSPGs are attributable to the structural divergence of the CS glycosaminoglycan (GAG) moieties. Because the expression profiles of the GAG chains of CSPGs change with developmental stage, aging, and disease progression, characterization of the GAG chains is essential to understand the functional roles of CSPGs. This chapter describes the basic protocols for GAG moiety-based immunochemical detection of CSPGs in biological samples in conjunction with CS disaccharide composition analysis.

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (C) #20K06551 (to T. M.) and Scientific Research (B) #20H03386 (to H. K.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Kobe Pharmaceutical University, Kobe, Japan

    Tadahisa Mikami & Hiroshi Kitagawa

Authors
  1. Tadahisa Mikami
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  2. Hiroshi Kitagawa
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Corresponding author

Correspondence to Hiroshi Kitagawa .

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Editors and Affiliations

  1. Biochemistry, Biochemical Analysis and Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece

    Nikos K. Karamanos

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mikami, T., Kitagawa, H. (2023). Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization of Chondroitin Sulfate Proteoglycans. In: Karamanos, N.K. (eds) Proteoglycans. Methods in Molecular Biology, vol 2619. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2946-8_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2946-8_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2945-1

  • Online ISBN: 978-1-0716-2946-8

  • eBook Packages: Springer Protocols

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