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In Vitro Generation of Murine Bone Marrow–Derived Dendritic Cells

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Dendritic Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2618))

Abstract

Dendritic cells (DCs) are mononuclear phagocytes of hematopoietic origin residing in lymphoid and nonlymphoid tissues. DCs are often referred as the sentinels of the immune system as they can sense pathogens and danger signals. Upon activation, DCs migrate to the draining lymph nodes and present antigens to naïve T cells to trigger adaptive immunity. Hematopoietic progenitors for DCs reside in the adult bone marrow (BM). Therefore, BM cell culture systems have been developed to generate large amounts of primary DCs in vitro conveniently enabling to analyze their developmental and functional features. Here, we review various protocols enabling to generate DCs in vitro from murine BM cells and discuss the cellular heterogeneity of each culture system.

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Authors and Affiliations

  1. PSL University, Institut Curie Research Center, INSERM U932, Center for Cancers Immunotherapy, Paris, France

    Yohan Gerber-Ferder & Julie Helft

  2. Université de Paris, Centre for Inflammation Research, CNRS ERL8252, INSERM1149, Paris, France

    Pierre Bourdely, Mathias Vetillard, Pierre Guermonprez & Julie Helft

  3. Université de Paris - Inserm - Cnrs, Institut Cochin, Paris, France

    Julie Helft

Authors
  1. Yohan Gerber-Ferder
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  2. Pierre Bourdely
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  3. Mathias Vetillard
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  4. Pierre Guermonprez
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  5. Julie Helft
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Corresponding author

Correspondence to Julie Helft .

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Editors and Affiliations

  1. UMR CNRS 5164 – Immunoconcept, Université de Bordeaux, Bordeaux, France

    Vanja Sisirak

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gerber-Ferder, Y., Bourdely, P., Vetillard, M., Guermonprez, P., Helft, J. (2023). In Vitro Generation of Murine Bone Marrow–Derived Dendritic Cells. In: Sisirak, V. (eds) Dendritic Cells. Methods in Molecular Biology, vol 2618. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2938-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2938-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2937-6

  • Online ISBN: 978-1-0716-2938-3

  • eBook Packages: Springer Protocols

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