Abstract
The main objective of this chapter is to determine inflammatory and anti-inflammatory cytokines in patients with or without preeclampsia by means of the enzyme-linked immunosorbent assay (ELISA) technique. In this chapter, 16 cell cultures were obtained from different patients admitted to the hospital with term vaginal delivery or cesarean section. Here, we describe the ability to quantify the presence of cytokines in cell culture supernatants. The supernatants of the cell cultures were collected and concentrated. The concentration of IL-6 and VEGF-R1 was measured by ELISA to determine the prevalence of alterations in the samples studied. We observed that the sensitivity of the kit allowed the detection of several cytokines in a range between 2 and 200 pg/mL. The test was performed using the ELISpot method (5×), which allowed a higher level of precision.
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References
Konstantinou GN (2017) Enzyme-linked immunosorbent assay (ELISA). Methods Mol Biol 1592:79–94. https://doi.org/10.1007/978-1-4939-6925-8_7
Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 72:4–15. https://doi.org/10.1016/j.peptides.2015.04.012. Epub 2015 Apr 20
Tighe PJ, Ryder RR, Todd I, Fairclough LC (2015) ELISA in the multiplex era: potentials and pitfalls. Proteomics Clin Appl 9(3–4):406–422. https://doi.org/10.1002/prca.201400130. Epub 2015 Mar 25. PMID: 25644123; PMCID: PMC6680274
Engvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8(9):871–874. https://doi.org/10.1016/0019-2791(71)90454-x
Ong DSY, Fragkou PC, Schweitzer VA et al (2021) How to interpret and use COVID-19 serology and immunology tests. Clin Microbiol Infect 27(7):981–986. https://doi.org/10.1016/j.cmi.2021.05.001. Epub 2021 May 8. PMID: 33975005; PMCID: PMC8106522
Beavis KG, Matushek SM, Abeleda APF et al (2020) Evaluation of the EUROIMMUN anti-SARS-CoV-2 ELISA assay for detection of IgA and IgG antibodies. J Clin Virol 129:104468. https://doi.org/10.1016/j.jcv.2020.104468. Epub 2020 May 23. PMID: 32485620; PMCID: PMC7255182
Kohl TO, Ascoli CA (2017) Direct competitive enzyme-linked immunosorbent assay (ELISA). Cold Spring Harb Protoc (7):pdb.prot093740. https://doi.org/10.1101/pdb.prot093740
Lin AV (2015) Indirect ELISA. Methods Mol Biol 1318:51–59. https://doi.org/10.1007/978-1-4939-2742-5_5
Hornbeck PV (2015) Enzyme-linked immunosorbent assays. Curr Protoc Immunol 110:2.1.1–2.1.23. https://doi.org/10.1002/0471142735.im0201s110
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Muñoz, O.B. (2023). Determination of Proinflammatory and Antiinflammatory Cytokines by ELISA Technique. In: Matson, R.S. (eds) ELISA. Methods in Molecular Biology, vol 2612. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2903-1_8
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DOI: https://doi.org/10.1007/978-1-0716-2903-1_8
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