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Immunoprecipitation Using Mono-ADP-Ribosylation-Specific Antibodies

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Poly(ADP-Ribose) Polymerase

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2609))

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Abstract

Immunoprecipitation is an essential methodology for enriching and purifying targeted proteins and peptides for in-depth analysis by any number of further techniques, from Western blotting to mass spectrometry (MS). Historically, the posttranslational modification ADP-ribosylation (ADPr) has been studied mainly in its polymerized form (poly-ADPr), but recent studies support the abundance and physiological relevance of mono-ADPr. Here, we describe several approaches to enrich mono-ADP-ribosylated proteins and peptides using mono-ADPr-specific antibodies, which can be tailored to a desired target and mode of downstream analysis.

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Acknowledgments

The work was funded by Germany’s Excellence Strategy (CECAD, EXC 2030-390661388 to I.M.) and the Max Planck Society, Germany. I.M. is supported by the EMBO Young Investigator Programme.

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Authors and Affiliations

  1. Research Group of Proteomics and ADP-ribosylation Signaling, Max Planck Institute for Biology of Ageing, Cologne, Germany

    Helen Dauben & Ivan Matic

  2. Cologne Excellence Cluster for Stress Responses in Ageing-Associated Diseases (CECAD), University of Cologne, Cologne, Germany

    Ivan Matic

Authors
  1. Helen Dauben
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  2. Ivan Matic
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Corresponding authors

Correspondence to Helen Dauben or Ivan Matic .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, University of North Dakota, Grand Forks, ND, USA

    Alexei V. Tulin

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Dauben, H., Matic, I. (2023). Immunoprecipitation Using Mono-ADP-Ribosylation-Specific Antibodies. In: Tulin, A.V. (eds) Poly(ADP-Ribose) Polymerase. Methods in Molecular Biology, vol 2609. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2891-1_9

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  • DOI: https://doi.org/10.1007/978-1-0716-2891-1_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2890-4

  • Online ISBN: 978-1-0716-2891-1

  • eBook Packages: Springer Protocols

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