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In Vivo Imaging of Naked and Microencapsulated Islet Cell Transplantation

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Type-1 Diabetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2592))

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Abstract

We describe step-by-step methods to label human pancreatic islet cells and murine insulinoma cells and their subsequent transplantation into type I diabetic mouse models with a focus on in vivo imaging using clinically applicable scanners. We also cover islets that are microencapsulated within alginate hydrogels loaded with imaging agents. By following these methods, it is possible to image cell grafts using T1-weighted and T2/T2*-weighted 1H magnetic resonance imaging (MRI), 19F MRI, computed tomography, ultrasound imaging, and bioluminescence imaging in vivo. Considering a myriad of factors that may affect the outcome of proper in vivo detection, we discuss potential issues that may be encountered during and after the process of labeling. The ultimate goal is to use these in vivo imaging approaches to determine and optimize naked and encapsulated islet cell survival, therapeutic function, and engraftment procedures.

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Author information

Authors and Affiliations

  1. Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Dian R. Arifin & Jeff W. M. Bulte

  2. Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Dian R. Arifin & Jeff W. M. Bulte

  3. Department of Chemical & Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jeff W. M. Bulte

  4. Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jeff W. M. Bulte

  5. Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jeff W. M. Bulte

Authors
  1. Dian R. Arifin
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  2. Jeff W. M. Bulte
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Corresponding author

Correspondence to Jeff W. M. Bulte .

Editor information

Editors and Affiliations

  1. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Anna Moore

  2. Precision Health Program, Michigan State University, East Lansing, MI, USA

    Ping Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Arifin, D.R., Bulte, J.W.M. (2023). In Vivo Imaging of Naked and Microencapsulated Islet Cell Transplantation. In: Moore, A., Wang, P. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 2592. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2807-2_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2807-2_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2806-5

  • Online ISBN: 978-1-0716-2807-2

  • eBook Packages: Springer Protocols

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