Abstract
Emerging studies, including ours, have revealed the novel essential roles of monoamine oxidase A (MAO A) in mediating the growth and progression of several types of cancers. Recently, we presented the first evidence of MAO A’s ability to promote cancer cell perineural invasion, the neoplastic invasion of nerves widely recognized as a significant route for cancer metastasis. Here, we describe a perineural invasion in vitro assay using a 3D coculture with a cancer cell line and an immortalized dorsal root ganglion neuronal cell line for rapid examination of MAO A’s roles in cancer-nerve cell crosstalk and evaluating the efficacy of MAO A inhibitors for disrupting perineural invasion. We also summarized the fundamental methods for determining MAO A’s effects on cancer cell proliferation in vitro and tumorigenesis in vivo.
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Acknowledgements
This work was supported by the DOD Prostate Cancer Research Program grants W81XWH-15-1-0493 and W81XWH-19-1-0279 and NIH/NCI grant R37CA233658 to B.J.W. and the DOD Prostate Cancer Research Program grant W81XWH-12-1-0282, the Tsai Family Fund and Boyd and Elsie Welin Professorship to J.C.S.
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Wu, B.J., Shih, J.C. (2023). In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers. In: Binda, C. (eds) Monoamine Oxidase. Methods in Molecular Biology, vol 2558. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2643-6_13
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DOI: https://doi.org/10.1007/978-1-0716-2643-6_13
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