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Metabolomics: Going Deeper, Going Broader, Going Further

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Cell-Wide Identification of Metabolite-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2554))

Abstract

Metabolomics is a continuously dynamic field of research that is driven by demanding research questions and technological advances alike. In this review we highlight selected recent and ongoing developments in the area of mass spectrometry-based metabolomics. The field of view that can be seen through the metabolomics lens can be broadened by adoption of separation techniques such as hydrophilic interaction chromatography and ion mobility mass spectrometry (going broader). For a given biospecimen, deeper metabolomic analysis can be achieved by resolving smaller entities such as rare cell populations or even single cells using nano-LC and spatially resolved metabolomics or by extracting more useful information through improved metabolite identification in untargeted metabolomic experiments (going deeper). Integration of metabolomics with other (omics) data allows researchers to further advance in the understanding of the complex metabolic and regulatory networks in cells and model organisms (going further). Taken together, diverse fields of research from mechanistic studies to clinics to biotechnology applications profit from these technological developments.

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Acknowledgments

The authors thank Stefan Christen for critical discussion of the manuscript.

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Author notes

  1. Sofia Moco and Joerg M. Buescher contributed equally with all other contributors.

Authors and Affiliations

  1. Molecular and Computational Toxicology, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, Amsterdam, Netherlands

    Sofia Moco

  2. Metabolomics Core Facility, Max Planck Institute of Immunobiology and Epigenetics, Freiburg im Breisgau, Germany

    Joerg M. Buescher

Authors
  1. Sofia Moco
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  2. Joerg M. Buescher
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Correspondence to Joerg M. Buescher .

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Editors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Aleksandra Skirycz

  2. Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany

    Marcin Luzarowski

  3. Interfaculty Institute of Cell Biology, University of Tübingen, Tübingen, Germany

    Jennifer C. Ewald

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Moco, S., Buescher, J.M. (2023). Metabolomics: Going Deeper, Going Broader, Going Further. In: Skirycz, A., Luzarowski, M., Ewald, J.C. (eds) Cell-Wide Identification of Metabolite-Protein Interactions. Methods in Molecular Biology, vol 2554. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2624-5_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2624-5_11

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  • Publisher Name: Humana, New York, NY

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