Abstract
In this chapter, we illustrate protocols for decellularization of the pig gallbladder with ionic biological detergent (0.5% and 1% sodium dodecyl sulfate) and nonionic biological detergent (0.5% and 1% Triton X-100) for different time intervals. The efficacy of the decellularization method for the preservation of the extracellular matrix scaffold structure and integrity was confirmed using histology, scanning electron microscopy, and DNA quantification. Treatment with 0.5% sodium dodecyl sulfate for 48 h resulted in complete decellularization of pig gallbladder.
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Sharma, A.K. et al. (2022). Tissue Scaffolds Derived from Pig Gallbladder. In: Kumar, N., Kumar, V., Shrivastava, S., Gangwar, A.K., Saxena, S. (eds) Tissue Scaffolds. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2425-8_8
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DOI: https://doi.org/10.1007/978-1-0716-2425-8_8
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