Abstract
Histological approach to long-term culture on collagen type I permits the evaluation of vasculogenic mimicry morphological features and the identification of endothelial-like cell-specific antigens. Here, we show the preparation of collagen type I solution, the embedding and the sections cutting of D17 osteosarcoma cells long-term culture, and then the hematoxylin and eosin (H&E) staining to identify endothelial-like structure. Moreover, we provide the protocols for periodic acid–Schiff (PAS) staining to evidence glycoproteins and CD31 immunohistochemistry to exclude the presence of this endothelial marker, as per definition by vasculogenic mimicry concept. This method allows to consider long-term culture as tissue, promoting the deeper study of vascular-like structures in vitro.
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Change history
11 September 2022
Correction to: Chapter 10 in: Emily Marques dos Reis and Fernanda Berti (eds.), Vasculogenic Mimicry, Methods in Molecular Biology Series, vol. 2514, https://doi.org/10.1007/978-1-0716-2403-6_10
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Massimini, M., Romanucci, M., Maria, R.D., Della Salda, L. (2022). Histological Evaluation of Long-Term Collagen Type I Culture. In: Marques dos Reis, E., Berti, F. (eds) Vasculogenic Mimicry. Methods in Molecular Biology, vol 2514. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2403-6_10
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DOI: https://doi.org/10.1007/978-1-0716-2403-6_10
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