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Liquid Chromatography-Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients

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Multiplex Biomarker Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2511))

Abstract

COVID-19 disease is caused by infection with the SARS-CoV-2 virus and is associated with a cytokine storm effect in some patients. This can lead to decreased ability of the host to cope with the infection and result in severe disease outcomes. Here, we present a protocol for isolation of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients followed by liquid chromatography-mass spectrometry (LC-MS) profiling to identify the affected molecules and molecular pathways. It is hoped that this will lead to the identification of potential biomarkers for monitoring the disease as well as treatment responses. This approach could also be used in the study of other respiratory viruses.

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Author information

Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

  2. Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge, UK

    Hassan Rahmoune

Authors
  1. Paul C. Guest
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  2. Hassan Rahmoune
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Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, University of Campinas, Campinas, Sao Paulo, Brazil

    Paul C. Guest

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Guest, P.C., Rahmoune, H. (2022). Liquid Chromatography-Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 2511. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2395-4_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2395-4_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2394-7

  • Online ISBN: 978-1-0716-2395-4

  • eBook Packages: Springer Protocols

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