Abstract
Heterologous expression is an easy and broadly applicable experimental approach widely used to investigate protein functions without the need to genetically manipulate the original host. The approach is used to obtain large quantities of the desired protein, which can be further analyzed from a biochemical, structural, and functional perspective. The expression system consists of three main components: (1) a foreign DNA sequence coding for the protein of interest; (2) a suitable expression vector; (3) a suitable host (bacterial, yeast, or mammalian cells) which does not encode or express the protein of interest. Here, we show how to apply an Escherichia coli-based expression system to overexpress protein encoding genes from marine microbes.
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Acknowledgments
This work was partially supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 948972), NSF grant IOS 19-51690, and NASA grant 80NSSC21K0485.
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Severino, A., Coppola, A., Correggia, M., Vetriani, C., Giovannelli, D., Cordone, A. (2022). From Sequences to Enzymes: Heterologous Expression of Genes from Marine Microbes. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_13
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DOI: https://doi.org/10.1007/978-1-0716-2313-8_13
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