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Recombineering

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Christopher R. Reisch

    1. Department of Microbiology and Cell Science, University of Florida, Gainesville, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2479)

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About this book

This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field. 


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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Scarless Recombineering of Phage in Lysogenic State

    • Maria Ababi, Matthew Tridgett, Alexander Osgerby, Alfonso Jaramillo
    Pages 1-9

  3. Lambda Red Recombineering of Bacteriophage in the Lysogenic State

    • Matthew Tridgett, Maria Ababi, Alfonso Jaramillo
    Pages 11-19

  4. Recombineering-Mediated Genome Editing in Burkholderiales Strains

    • Xue Wang, Jiaqi Liu, Wentao Zheng, Youming Zhang, Xiaoying Bian
    Pages 21-36

  5. High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy

    • Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
    Pages 37-52

  6. Creation of Markerless Genome Modifications in a Nonmodel Bacterium by Fluorescence-Aided Recombineering

    • Piyush Behari Lal, Fritz Wells, Patricia J. Kiley
    Pages 53-70

  7. Plant Gene Modification by BAC Recombineering

    • Zhoubo Hu, Ajit Ghosh, Csaba Koncz
    Pages 71-84

  8. Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling

    • Joakim Näsvall
    Pages 85-104

  9. Genome Editing in Klebsiella pneumoniae Using CRISPR/Cas9 Technology

    • Zhipeng Wang, Yu Wang, Quanjiang Ji
    Pages 105-117

  10. Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System

    • Adam J. Ellington, Christopher R. Reisch
    Pages 119-133

  11. Recombineering in Staphylococcus aureus

    • Kelsi Penewit, Stephen J. Salipante
    Pages 135-157

  12. Genome Editing Methods for Bacillus subtilis

    • Katherine J. Wozniak, Lyle A. Simmons
    Pages 159-174

  13. Highly Efficient Genome Editing in Clostridium difficile Using the CRISPR-Cpf1 System

    • Wei Hong, Jie Zhang, Guzhen Cui, Qian Zhou, Pixiang Wang, Yi Wang
    Pages 175-187

  14. Genome Editing of Corynebacterium glutamicum Using CRISPR-Cpf1 System

    • Zhiqiang Wen, Fenghui Qian, Jiao Zhang, Yu Jiang, Sheng Yang
    Pages 189-206

  15. CRISPR/Cas-Mediated Genome Editing of Streptomyces

    • Lee Ling Tan, Elena Heng, Nadiah Zulkarnain, Wan-Chi Hsiao, Fong Tian Wong, Mingzi M. Zhang
    Pages 207-225

  16. Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum

    • Tao Xu, Xuanyu Tao, Megan L. Kempher, Jizhong Zhou
    Pages 227-243

  17. Genome Editing with Cas9 in Lactobacilli

    • Justin M. Vento, Chase L. Beisel
    Pages 245-261

  18. Back Matter

    Pages 263-264
    Download chapter PDF
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Editors and Affiliations

  • Department of Microbiology and Cell Science, University of Florida, Gainesville, USA

    Christopher R. Reisch

Bibliographic Information

  • Book Title: Recombineering

  • Book Subtitle: Methods and Protocols

  • Editors: Christopher R. Reisch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2233-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2232-2Published: 18 May 2022

  • Softcover ISBN: 978-1-0716-2235-3Published: 19 May 2023

  • eBook ISBN: 978-1-0716-2233-9Published: 18 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 264

  • Number of Illustrations: 7 b/w illustrations, 50 illustrations in colour

  • Topics: Microbiology, Microbial Genetics and Genomics

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