Abstract
The accumulation of the cell wall component callose at plasmodesmata (PD) is crucial for the regulation of symplastic intercellular transport in plants. Here we describe protocols to fluorescently image callose in sectioned plant tissue using monoclonal antibodies. This protocol achieves high-resolution images by the fixation, embedding, and sectioning of plant material to expose internal cell walls. By using this protocol in combination with high-resolution confocal microscopy, we can detect PD callose in a variety of plant tissues and species.
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References
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Acknowledgments
Financial support for this research was provided by the Leverhulme Trust Grant RPG-2016-136.
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Amsbury, S., Benitez-Alfonso, Y. (2022). Immunofluorescence Detection of Callose in Plant Tissue Sections. In: Benitez-Alfonso, Y., Heinlein, M. (eds) Plasmodesmata. Methods in Molecular Biology, vol 2457. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2132-5_10
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DOI: https://doi.org/10.1007/978-1-0716-2132-5_10
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