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Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions

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Dengue Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2409))

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Abstract

Yeast two-hybrid (Y2H) systems are one of the principal choices for identifying novel binary protein-protein interactions (PPIs). Since its development, it has contributed for the discovery of several PPIs between pathogens and host, allowing not only a comprehensive look at the disease pathogenesis but also for therapeutic strategies. Identification of viral-host PPIs that impact on viral replication and pathogenesis can lead to new advances in antiviral therapies such as the development of drug candidates and vaccine design. In this chapter, we revise the Y2H key parameters necessary for screening PPIs and discuss the possible approaches for using this technique to identify novel dengue-host protein interactions.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA

    Jonas Nascimento Conde

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  1. Jonas Nascimento Conde
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Correspondence to Jonas Nascimento Conde .

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Editors and Affiliations

  1. Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Ronaldo Mohana-Borges

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Conde, J.N. (2022). Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions. In: Mohana-Borges, R. (eds) Dengue Virus. Methods in Molecular Biology, vol 2409. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1879-0_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1879-0_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1878-3

  • Online ISBN: 978-1-0716-1879-0

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