Abstract
The human decidua basalis, main uterine mucosa during pregnancy, provides an ex vivo model for studying natural protection of macrophages against HIV-1 infection at the mucosal level. Beyond pregnancy, the decidua constitutes also a valuable tool to assess tissue-resident macrophage infection. Here, we provide a detailed protocol for decidual macrophage purification and tissue infection.
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References
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Acknowledgments
We would like to thank all the women donors who gave their informed consent and the clinical personnel for providing the samples. We would also like to thank INSERM, ANRS, and Sidaction for their financial support.
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Jabrane-Ferrat, N., El Costa, H. (2022). Decidua Basalis: An Ex Vivo Model to Study HIV-1 Infection During Pregnancy and Beyond. In: Poli, G., Vicenzi, E., Romerio, F. (eds) HIV Reservoirs. Methods in Molecular Biology, vol 2407. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1871-4_15
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DOI: https://doi.org/10.1007/978-1-0716-1871-4_15
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