Abstract
microRNA capture affinity technology (miR-CATCH) uses affinity capture biotinylated antisense oligonucleotides to co-purify a target transcript together with all its endogenously bound miRNAs. The miR-CATCH assay is performed to investigate miRNAs bound to a specific mRNA. This method allows to have a total vision of miRNAs bound not only to the 3′UTR but also to the 5′UTR and Coding Region of target messenger RNAs (mRNAs).
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Acknowledgments
We wish to thank Catherine M. Greene (Royal College of Surgeons in Ireland, Dublin) for having hosted Francesca Fontana in her laboratory in the Fall of 2013, and Sebastian Vencken (Royal College of Surgeons in Ireland, Dublin) for having taught Francesca the miR-CATCH protocol. We also wish to thank Francesca Fontana for having shared with the rest of the Denti Lab her knowledge of the miR-CATCH methodology.
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Zeni, A., Grasso, M., Denti, M.A. (2022). Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH). In: Dassi, E. (eds) Post-Transcriptional Gene Regulation. Methods in Molecular Biology, vol 2404. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1851-6_11
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DOI: https://doi.org/10.1007/978-1-0716-1851-6_11
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