Abstract
We demonstrated the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. This chapter provides materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord and human dorsal root ganglia isolated from brain-dead patients who had agreed before passing to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Becker CG, Becker T, Hugnot JP (2018) The spinal ependymal zone as a source of endogenous repair cells across vertebrates. Prog Neurobiol 170:67–80. https://doi.org/10.1016/j.pneurobio.2018.04.002
Hamilton LK, Truong MK, Bednarczyk MR, Aumont A, Fernandes KJ (2009) Cellular organization of the central canal ependymal zone, a niche of latent neural stem cells in the adult mammalian spinal cord. Neuroscience 164(3):1044–1056. https://doi.org/10.1016/j.neuroscience.2009.09.006
Stenudd M, Sabelstrom H, Frisen J (2015) Role of endogenous neural stem cells in spinal cord injury and repair. JAMA Neurol 72(2):235–237. https://doi.org/10.1001/jamaneurol.2014.2927
Dromard C, Guillon H, Rigau V, Ripoll C, Sabourin JC, Perrin FE, Scamps F, Bozza S, Sabatier P, Lonjon N, Duffau H, Vachiery-Lahaye F, Prieto M, Tran Van Ba C, Deleyrolle L, Boularan A, Langley K, Gaviria M, Privat A, Hugnot JP, Bauchet L (2008) Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro. J Neurosci Res 86(9):1916–1926. https://doi.org/10.1002/jnr.21646
Ghazale H, Ripoll C, Leventoux N, Jacob L, Azar S, Mamaeva D, Glasson Y, Calvo CF, Thomas JL, Meneceur S, Lallemand Y, Rigau V, Perrin FE, Noristani HN, Rocamonde B, Huillard E, Bauchet L, Hugnot JP (2019) RNA profiling of the human and mouse spinal cord stem cell niches reveals an embryonic-like regionalization with MSX1(+) roof-plate-derived cells. Stem Cell Rep 12(5):1159–1177. https://doi.org/10.1016/j.stemcr.2019.04.001
Mamaeva D, Ripoll C, Bony C, Teigell M, Perrin FE, Rothhut B, Bieche I, Lidereau R, Privat A, Rigau V, Guillon H, Vachiery-Lahaye F, Noel D, Bauchet L, Hugnot JP (2011) Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord. BMC Neurosci 12:99. https://doi.org/10.1186/1471-2202-12-99
Perrin FE, Gerber YN, Teigell M, Lonjon N, Boniface G, Bauchet L, Rodriguez JJ, Hugnot JP, Privat AM (2011) Anatomical study of serotonergic innervation and 5-HT(1A) receptor in the human spinal cord. Cell Death Dis 2:e218. https://doi.org/10.1038/cddis.2011.98
Agence de la Biomédecine (2019) Recommandations pour la pratique professionnelle. Prise en charge des patients en vue d’un prélèvement d’organes (2019). https://www.agence-biomedecine.fr/IMG/pdf/prise_en_charge_des_patient_en_vue_d_un_prelevement_d_organes_-_2019_-_version_longue.pdf
Tixier D, Barrou B (2007) Recommandations techniques pour le prélèvement des organes et des tissus sur donneurs en état de mort encéphalique. https://www.agence-biomedecine.fr/IMG/pdf/recommandations-techniques-concernant-le-donneur-en-etat-de_mort-encephalique.pdf
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bauchet, L. et al. (2022). Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord. In: Deleyrolle, L.P. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 2389. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1783-0_10
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1783-0_10
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1782-3
Online ISBN: 978-1-0716-1783-0
eBook Packages: Springer Protocols