Abstract
Recent advances in single-cell technologies have made it possible to gather increased amounts of information about even rare cell subtypes. In particular, mass cytometry is able to assess the expression of 30–50 proteins on millions of cells. Within CD4 T-cells, T-follicular helper cells (Tfh) and their regulatory counterpart, T-follicular regulatory cells (Tfr), localize to the B-cell follicle and have specialized roles in the maintenance and regulation of B-cell antibody production. The frequency of Tfh and Tfr in circulation has also been associated with ongoing antibody responses.
In this chapter, we detail methods to analyze the frequency and phenotype of the populations of Tfh and Tfr found in humans by mass cytometry.
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Acknowledgments
The authors would like to thank Rika Ishii for technical support and Dr. Ee Lyn Lim for proofreading the document.
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Wing, J.B., Sakaguchi, S. (2022). Using Mass Cytometry to Address Tfh and Tfr Heterogeneity. In: Graca, L. (eds) T-Follicular Helper Cells. Methods in Molecular Biology, vol 2380. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1736-6_5
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DOI: https://doi.org/10.1007/978-1-0716-1736-6_5
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