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Screening for Anticancer Activity: Trypan Blue Exclusion Assay

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Methods in Actinobacteriology

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Anti-cancer activity of different bacterial (actinobacterial extracts) metabolites were detrmined by cytotoxicity assay such as MTT assay, trypan blue exclusion assay, and phase contrast inverted microscope morphological assessment. IC50 (the concentration that inhibited cell growth by 50 percent) was calculated for the bacterial metabolite.

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Author information

Authors and Affiliations

  1. Departmentof Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan

    InamulHasan Madar

  2. Departmentof Computer Science, Faculty of Science, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Ghazala Sultan

  3. Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do, South Korea

    Ramachandran Chelliah & Deog-Hwan Oh

Authors
  1. InamulHasan Madar
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  2. Ghazala Sultan
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  3. Ramachandran Chelliah
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  4. Deog-Hwan Oh
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Editor information

Editors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Dhanasekaran Dharumadurai

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Madar, I., Sultan, G., Chelliah, R., Oh, DH. (2022). Screening for Anticancer Activity: Trypan Blue Exclusion Assay. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_59

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  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_59

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

  • eBook Packages: Springer Protocols

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