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Biosynthesis and Characterization of Silver Nanoparticles from Actinobacteria

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Methods in Actinobacteriology

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

An efficient biosynthetic process for the rapid production of nanoparticles would enable the development of “microbial nanotechnology” for mass-scale production. The present protocol focuses on the methodology to synthesize and characterize the silver nanoparticle (AgNPs) using actinobacterial strains. UV-Vis spectroscopy and High Resolution Transmission electron microscopy (HR-TEM) are proposed to be used to characterize the synthesized AgNPs. X-ray diffraction (XRD) of the nanoparticles dispersion will confirm the presence or absence of elemental silver signal peaks.

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References

  1. Składanowski M, Wypij M, Laskowski D, Golinska P, Dahm H, Rai M (2017) Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil with special reference to its antibacterial activity against pathogens. J Clust Sci 28(1):59–79

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    R. T. V. Vimala & S. Sridharan

  2. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, People’s Republic of China

    G. Rajivgandhi & Wen-Jun Li

  3. Marine Pharmacology and Toxicology Laboratory, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    G. Rajivgandhi, G. Ramachandran, C. Chenthis Kanisha & N. Manoharan

  4. Department of Petrochemical Technology, Anna University-BIT Campus, Tiruchirappalli, Tamil Nadu, India

    M. Jayapriya

Authors
  1. R. T. V. Vimala
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  2. G. Rajivgandhi
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  3. S. Sridharan
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  4. M. Jayapriya
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  5. G. Ramachandran
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  6. C. Chenthis Kanisha
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  7. N. Manoharan
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  8. Wen-Jun Li
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Editor information

Editors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    Dhanasekaran Dharumadurai

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cite this protocol

Vimala, R.T.V. et al. (2022). Biosynthesis and Characterization of Silver Nanoparticles from Actinobacteria. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_103

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  • DOI: https://doi.org/10.1007/978-1-0716-1728-1_103

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1727-4

  • Online ISBN: 978-1-0716-1728-1

  • eBook Packages: Springer Protocols

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