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Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells

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Cytoskeleton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2364))

Abstract

Budding yeast, Saccharomyces cerevisiae, is an appealing model organism to study the organization and function of the actin cytoskeleton. With the advent of techniques to perform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fluorescence microscopy.

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Acknowledgments

We thank the members of the Pon laboratory for support and invaluable advice. This work was supported by awards from the National Institutes of Health (NIH) (GM122589 and AG051047) and Muscular Dystrophy Association (MDA 314107) to LAP; the NIH (AG055326 and DK7647) to CNS; and awards from the National Institute of Aging (NIA) (1K99AG065200-01A1) to RHS. The Confocal and Specialized Microscopy Shared Resource is in the Herbert Irving Comprehensive Cancer Center at Columbia University Medical Center, which is supported in part by an award from the NIH/NCI (5 P30 CA13696).

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Authors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA

    Cierra N. Sing, Emily J. Yang & Istvan R. Boldogh

  2. Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA

    Ryo Higuchi-Sanabria

  3. Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA

    Liza A. Pon, Istvan R. Boldogh & Theresa C. Swayne

  4. Department of Pathology and Cell Biology, Columbia University, New York, NY, USA

    Liza A. Pon

Authors
  1. Cierra N. Sing
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  2. Emily J. Yang
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  3. Ryo Higuchi-Sanabria
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  4. Liza A. Pon
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  5. Istvan R. Boldogh
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  6. Theresa C. Swayne
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Corresponding author

Correspondence to Liza A. Pon .

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Editors and Affiliations

  1. Professor Emeritus, Brooklyn College – CUNY, Brooklyn, NY, USA

    Ray H. Gavin

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sing, C.N., Yang, E.J., Higuchi-Sanabria, R., Pon, L.A., Boldogh, I.R., Swayne, T.C. (2022). Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells. In: Gavin, R.H. (eds) Cytoskeleton . Methods in Molecular Biology, vol 2364. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1661-1_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1661-1_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1660-4

  • Online ISBN: 978-1-0716-1661-1

  • eBook Packages: Springer Protocols

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