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Proteomic Tools for the Analysis of Cytoskeleton Proteins

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Cytoskeleton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2364))

Abstract

Proteomic analyses have become an essential part of the toolkit of the molecular biologist, given the widespread availability of genomic data and open source or freely accessible bioinformatics software. Tools are available for detecting homologous sequences, recognizing functional domains, and modeling the three-dimensional structure for any given protein sequence, as well as for predicting interactions with other proteins or macromolecules. Although a wealth of structural and functional information is available for many cytoskeletal proteins, with representatives spanning all of the major subfamilies, the majority of cytoskeletal proteins remain partially or totally uncharacterized. Moreover, bioinformatics tools provide a means for studying the effects of synthetic mutations or naturally occurring variants of these cytoskeletal proteins. This chapter discusses various freely available proteomic analysis tools, with a focus on in silico prediction of protein structure and function. The selected tools are notable for providing an easily accessible interface for the novice while retaining advanced functionality for more experienced computational biologists.

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Authors and Affiliations

  1. Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY, USA

    Carlos Barreto, Andriele Silva, Eliza Wiech, Antonio Lopez, Avdar San & Shaneen Singh

  2. The Biochemistry Ph.D. Program, The Graduate Center of the City University of New York, New York, NY, USA

    Andriele Silva, Avdar San & Shaneen Singh

  3. The Biology Ph.D. program, The Graduate Center of the City University of New York, New York, NY, USA

    Shaneen Singh

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  1. Carlos Barreto
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  2. Andriele Silva
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  3. Eliza Wiech
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  4. Antonio Lopez
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  5. Avdar San
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  6. Shaneen Singh
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Correspondence to Shaneen Singh .

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  1. Professor Emeritus, Brooklyn College – CUNY, Brooklyn, NY, USA

    Ray H. Gavin

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Barreto, C., Silva, A., Wiech, E., Lopez, A., San, A., Singh, S. (2022). Proteomic Tools for the Analysis of Cytoskeleton Proteins. In: Gavin, R.H. (eds) Cytoskeleton . Methods in Molecular Biology, vol 2364. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1661-1_19

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  • DOI: https://doi.org/10.1007/978-1-0716-1661-1_19

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