Abstract
Using Drosophila actin-binding protein Dunc-115 as an example, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single cell level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.
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Acknowledgments
We would like to thank the Bloomington Stock Center for providing fly strains, Junaid Syed for assistance, and Ray Gavin for reviewing the manuscript. This work was supported in part by a PSC-CUNY grant.
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He, Q., Roblodowski, C. (2022). Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila . In: Gavin, R.H. (eds) Cytoskeleton . Methods in Molecular Biology, vol 2364. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1661-1_17
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DOI: https://doi.org/10.1007/978-1-0716-1661-1_17
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1660-4
Online ISBN: 978-1-0716-1661-1
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