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Plant Phosphoproteomics pp 169–177Cite as

2-D DIGE Combined with Pro-Q Diamond Staining for the Identification of Protein Phosphorylation for Chlamydomonas reinhardtii : A Successful Approach

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2358))

Abstract

The green alga Chlamydomonas reinhardtii is an extremely useful model organism, and protein phosphorylation is an extremely important posttranslational modification. We have established the protocol 2-D difference gel electrophoresis (DIGE), combined with the fluorescence staining with Pro-Q Diamond, that successfully detects subtle changes of spot mobility caused by protein phosphorylation between Chlamydomonas samples.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (31972934).

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Authors and Affiliations

  1. College of Life Sciences, Capital Normal University, Beijing, China

    Li Li

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  1. Li Li
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Correspondence to Li Li .

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Editors and Affiliations

  1. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, China

    Xu Na Wu

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Li, L. (2021). 2-D DIGE Combined with Pro-Q Diamond Staining for the Identification of Protein Phosphorylation for Chlamydomonas reinhardtii : A Successful Approach. In: Wu, X.N. (eds) Plant Phosphoproteomics. Methods in Molecular Biology, vol 2358. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1625-3_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1625-3_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1624-6

  • Online ISBN: 978-1-0716-1625-3

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