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The New Frontier of Three-Dimensional Culture Models to Scale-Up Cancer Research

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Physical Exercise and Natural and Synthetic Products in Health and Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2343))

Abstract

In vitro cancer research models require the utmost accuracy and precision to effectively investigate physiological pathways and mechanisms, as well as test the therapeutic efficacy of anticancer drugs. Although two-dimensional (2D) cell culture models have been the traditional hallmark of cancer research, increasing evidence suggests 2D tumor models cannot accurately recapitulate complex aspects of tumor cells and drug responses. Three-dimensional (3D) cell cultures, however, are more physiologically relevant in oncology as they model the cancer network and microenvironment better, allowing for development and assessment of natural products and other anticancer drugs. The present review outlines unprecedented ways in which multicellular spheroid models, organoid models, hydrogel models, microfluidic devices, microfiber scaffold models, and tissue-engineered scaffold models are used in this research. The future of cancer research lies within 3D cell cultures, and as this approach improves, cancer research will continue to advance.

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Abbreviations

2D:

Two-dimensional

3D:

Three-dimensional

3DP:

3D printing

AQP5:

Aquaporin 5

BGD:

Bendamustine, gemcitabine, dexamethasone

CPDD:

Cisplatin

CSC:

Cancer stem cells

ECM:

Extracellular matrix

EIT:

Electrical impedance tomography

EMT:

Epithelial to mesenchyme transition

FEM:

Finite element method

fFn:

Fibrillar fibronectin

Fn:

Fibronectin

GelMA:

Gelatin methacryloyl

GSCs:

Glioblastoma stem cells

HCC:

Hepatocellular carcinoma

HTS:

High-throughput screening

HUVECs:

Human umbilical vein endothelial cells

Micro-CT:

X-ray microcomputed tomography

MPM:

Malignant pleural mesothelioma

NAP1:

Nck-associated protein 1

NSCLC:

Non-small-cell lung cancer

OC:

Ovarian cancer

OXY:

Oxyresveratrol

PDAC:

Pancreatic ductal adenocarcinoma

PDGFBB:

Platelet-derived growth factor BB

PEG:

Polyethylene glycol

PEM:

Pemetrexed

PET:

Polyethylene terephthalate

PLA:

Polylactic acid

PLGA:

Poly(lactide-co-glycolide)

PVA:

Poly(vinyl acetate)

SCAPs:

Stem cells from apical papilla

TE:

Tissue engineering

TRPV4:

Transient receptor potential cation channel

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Acknowledgments

This work was supported in part by NIH grants R01DE028351 and R03DE028387 and CURS Summer Scholars (to Y. Teng).

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Authors and Affiliations

  1. Department of Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University, Augusta, GA, USA

    Caleb Jensen & Yong Teng

  2. Department of Pediatrics, Emory Children’s Center, Emory University, Atlanta, GA, USA

    Chloe Shay

  3. Georgia Cancer Center, Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA, USA

    Yong Teng

  4. Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University, Atlanta, GA, USA

    Yong Teng

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  1. Caleb Jensen
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  2. Chloe Shay
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  3. Yong Teng
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Correspondence to Yong Teng .

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  1. Charlesworth House, Debden, Essex, UK

    Paul C. Guest

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Jensen, C., Shay, C., Teng, Y. (2022). The New Frontier of Three-Dimensional Culture Models to Scale-Up Cancer Research. In: Guest, P.C. (eds) Physical Exercise and Natural and Synthetic Products in Health and Disease. Methods in Molecular Biology, vol 2343. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1558-4_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1558-4_1

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