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Cell Cycle Oscillators

Methods and Protocols

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Amanda S. Coutts

    1. School of Science and Technology, Nottingham Trent University, Nottingham, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Louise Weston

    1. Scientific Writer, Newcastle upon Tyne, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2329)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Cell Cycle Control: A System of Interlinking Oscillators

    • Randy Y. C. Poon
    Pages 1-18

  3. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity

    • Matthew P. Swaffer
    Pages 19-27

  4. Real-Time Monitoring of APC /C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts

    • Julia Kamenz, Renping Qiao, Qiong Yang, James E. Ferrell Jr
    Pages 29-38

  5. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts

    • Morgan Pellerano, May C. Morris
    Pages 39-50

  6. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry

    • Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, Mark C. Hall
    Pages 51-70

  7. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots

    • Hirotomo Takatsuka, Masaaki Umeda
    Pages 71-80

  8. Construction of a Full-Length 3′UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs

    • Dominika Kaźmierczak, Per Hydbring
    Pages 81-94

  9. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis

    • Mardo Kõivomägi
    Pages 95-109

  10. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block

    • Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, Cristina Montiel-Duarte
    Pages 111-121

  11. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition

    • Pawan Singh, Lenka Halova, Iain Michael Hagan
    Pages 123-142

  12. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System

    • Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P. Enrico et al.
    Pages 143-164

  13. EDU (5-Ethynyl-2′-Deoxyuridine)-Coupled Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle

    • João A. Ferreira, Marco Neves, Miguel Alpalhão, Pedro Pereira, Daniela Cunha, Fernando Ferreira et al.
    Pages 165-177

  14. High-Resolution Analysis of Centrosome Behavior During Mitosis

    • Vanessa Nunes, Margarida Dantas, Joana T. Lima, Jorge G. Ferreira
    Pages 179-194

  15. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells

    • Jonathan M. Geisinger, Tim Stearns
    Pages 195-204

  16. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle

    • Isha Nasa, Greg B. Moorhead, Arminja N. Kettenbach
    Pages 205-221

  17. Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell Cycle Indicator (Fucci)

    • Atsushi Kaida, Masahiko Miura
    Pages 223-236

  18. Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody

    • Yuko Sato, Hiroshi Kimura
    Pages 237-247

  19. Analyzing Centrioles and Cilia by Expansion Microscopy

    • Dong Kong, Jadranka Loncarek
    Pages 249-263

  20. Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae

    • Deniz Pirincci Ercan, Frank Uhlmann
    Pages 265-276Open Access
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Keywords

About this book

This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology and biochemical techniques for studying oscillatory networks and to share these across the research community to facilitate further progress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cell Cycle Oscillators: Methods and Protocols, Second Edition serves as an invaluable guide for those new to the field as well as the more experienced scientist.




Chapters 19 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • School of Science and Technology, Nottingham Trent University, Nottingham, UK

    Amanda S. Coutts

  • Scientific Writer, Newcastle upon Tyne, UK

    Louise Weston

Bibliographic Information

  • Book Title: Cell Cycle Oscillators

  • Book Subtitle: Methods and Protocols

  • Editors: Amanda S. Coutts, Louise Weston

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1538-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1537-9Published: 04 June 2021

  • Softcover ISBN: 978-1-0716-1540-9Published: 05 June 2022

  • eBook ISBN: 978-1-0716-1538-6Published: 03 June 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 340

  • Number of Illustrations: 10 b/w illustrations, 74 illustrations in colour

  • Topics: Cell Biology

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