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High-Throughput Measurement for Toxic Effects of Metal Mixtures in Caenorhabditis elegans

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Environmental Toxicology and Toxicogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2326))

Abstract

The contamination of heavy metals, a class of naturally occurring and persistent toxicants, has become a major public health concern due to increasing industrial and anthropogenic activities. The use of COPAS Biosort, a flow cytometer capable of measuring thousands of nematodes in minutes via high-throughput assays, has been widely applied in C. elegans studies for assessing toxicity of individual metals; however, such application yet to be seen for metals or other chemical mixtures. In the present protocol, we investigated toxic effects of individual metals, Cd, Pb, and Mn, as well as their binary and ternary mixtures, using nematode C. elegans. The toxic outcomes, including effects on growth, reproduction, and feeding behavior, were measured using high-throughput platform analysis (COAPS Biosort).

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References

  1. Byber K, Lison D, Verougstraete V, Dressel H, Hotz P (2016) Cadmium or cadmium compounds and chronic kidney disease in workers and the general population: a systematic review. Crit Rev Toxicol 46(3):191–240. https://doi.org/10.3109/10408444.2015.1076375

    Article  CAS  PubMed  Google Scholar 

  2. Cinnirella S, Hedgecock IM, Sprovieri F (2014) Heavy metals in the environment: sources, interactions and human health. Environ Sci Pollut Res Int 21(6):3997–3998. https://doi.org/10.1007/s11356-013-2486-z

    Article  PubMed  Google Scholar 

  3. Jomova K, Valko M (2011) Advances in metal-induced oxidative stress and human disease. Toxicology 283(2–3):65–87. https://doi.org/10.1016/j.tox.2011.03.001

    Article  CAS  PubMed  Google Scholar 

  4. Lim JT, Tan YQ, Valeri L, Lee J, Geok PP, Chia SE, Ong CN, Seow WJ (2019) Association between serum heavy metals and prostate cancer risk—a multiple metal analysis. Environ Int 132:105109. https://doi.org/10.1016/j.envint.2019.105109

    Article  CAS  PubMed  Google Scholar 

  5. Rehman K, Fatima F, Waheed I, Akash MSH (2018) Prevalence of exposure of heavy metals and their impact on health consequences. J Cell Biochem 119(1):157–184. https://doi.org/10.1002/jcb.26234

    Article  CAS  PubMed  Google Scholar 

  6. Caito S, Aschner M (2015) Neurotoxicity of metals. Handb Clin Neurol 131:169–189. https://doi.org/10.1016/b978-0-444-62627-1.00011-1

    Article  PubMed  Google Scholar 

  7. Balistrieri LS, Mebane CA (2014) Predicting the toxicity of metal mixtures. Sci Total Environ 466–467:788–799. https://doi.org/10.1016/j.scitotenv.2013.07.034

    Article  CAS  PubMed  Google Scholar 

  8. Adebambo OA, Ray PD, Shea D, Fry RC (2015) Toxicological responses of environmental mixtures: environmental metal mixtures display synergistic induction of metal-responsive and oxidative stress genes in placental cells. Toxicol Appl Pharmacol 289(3):534–541. https://doi.org/10.1016/j.taap.2015.10.005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Cobbina SJ, Chen Y, Zhou Z, Wu X, Feng W, Wang W, Mao G, Xu H, Zhang Z, Wu X, Yang L (2015) Low concentration toxic metal mixture interactions: effects on essential and non-essential metals in brain, liver, and kidneys of mice on sub-chronic exposure. Chemosphere 132:79–86. https://doi.org/10.1016/j.chemosphere.2015.03.013

    Article  CAS  PubMed  Google Scholar 

  10. Leung MC, Williams PL, Benedetto A, Au C, Helmcke KJ, Aschner M, Meyer JN (2008) Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology. Toxicol Sci 106(1):5–28. https://doi.org/10.1093/toxsci/kfn121

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Meyer D, Williams PL (2014) Toxicity testing of neurotoxic pesticides in Caenorhabditis elegans. J Toxicol Environ Health B Crit Rev 17(5):284–306. https://doi.org/10.1080/10937404.2014.933722

    Article  CAS  PubMed  Google Scholar 

  12. Hunt PR (2017) The C. elegans model in toxicity testing. J Appl Toxicol 37(1):50–59. https://doi.org/10.1002/jat.3357

    Article  CAS  PubMed  Google Scholar 

  13. Queiros L, Pereira JL, Goncalves FJM, Pacheco M, Aschner M, Pereira P (2019) Caenorhabditis elegans as a tool for environmental risk assessment: emerging and promising applications for a “nobelized worm”. Crit Rev Toxicol 49(5):411–429. https://doi.org/10.1080/10408444.2019.1626801

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Pulak R (2006) Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system. Methods Mol Biol 351:275–286. https://doi.org/10.1385/1-59745-151-7:275

    Article  PubMed  Google Scholar 

  15. Boyd WA, Smith MV, Kissling GE, Freedman JH (2010) Medium- and high-throughput screening of neurotoxicants using C. elegans. Neurotoxicol Teratol 32(1):68–73. https://doi.org/10.1016/j.ntt.2008.12.004

    Article  CAS  PubMed  Google Scholar 

  16. Boyd WA, McBride SJ, Rice JR, Snyder DW, Freedman JH (2010) A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay. Toxicol Appl Pharmacol 245(2):153–159. https://doi.org/10.1016/j.taap.2010.02.014

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Boyd WA, Smith MV, Freedman JH (2012) Caenorhabditis elegans as a model in developmental toxicology. Methods Mol Biol 889:15–24. https://doi.org/10.1007/978-1-61779-867-2_3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Chou TC (2006) Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev 58(3):621–681. https://doi.org/10.1124/pr.58.3.10

    Article  CAS  PubMed  Google Scholar 

  19. Wang Y, Chen C, Qian Y, Zhao X, Wang Q, Kong X (2015) Toxicity of mixtures of lambda-cyhalothrin, imidacloprid and cadmium on the earthworm Eisenia fetida by combination index (CI)-isobologram method. Ecotoxicol Environ Saf 111:242–247. https://doi.org/10.1016/j.ecoenv.2014.10.015

    Article  CAS  PubMed  Google Scholar 

  20. Rodea-Palomares I, Petre AL, Boltes K, Leganes F, Perdigon-Melon JA, Rosal R, Fernandez-Pinas F (2010) Application of the combination index (CI)-isobologram equation to study the toxicological interactions of lipid regulators in two aquatic bioluminescent organisms. Water Res 44(2):427–438. https://doi.org/10.1016/j.watres.2009.07.026

    Article  CAS  PubMed  Google Scholar 

  21. Williams PL, Dusenbery DB (1990) Aquatic toxicity testing using the nematode, Caenorhabditis elegans. Environ Toxicol Chem 9(10):1285–1290

    Article  CAS  Google Scholar 

  22. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77(1):71–94

    Article  CAS  Google Scholar 

  23. Chou TC, Talalay P (1984) Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzym Regul 22:27–55

    Article  CAS  Google Scholar 

  24. Boyd WA, Smith MV, Kissling GE, Rice JR, Snyder DW, Portier CJ, Freedman JH (2009) Application of a mathematical model to describe the effects of chlorpyrifos on Caenorhabditis elegans development. PLoS One 4(9):e7024

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA, USA

    Kathy S. Xue & Lili Tang

Authors
  1. Kathy S. Xue
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  2. Lili Tang
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Corresponding author

Correspondence to Lili Tang .

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Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Xiaoping Pan

  2. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Xue, K.S., Tang, L. (2021). High-Throughput Measurement for Toxic Effects of Metal Mixtures in Caenorhabditis elegans. In: Pan, X., Zhang, B. (eds) Environmental Toxicology and Toxicogenomics. Methods in Molecular Biology, vol 2326. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1514-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1514-0_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1513-3

  • Online ISBN: 978-1-0716-1514-0

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