Abstract
For structural, biochemical, or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows for efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the “tRNA scaffold” method to RNA–protein coexpression in order to express and purify RNA by affinity in native condition. As a proof of concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
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El Khouri, M. et al. (2021). Coexpression and Copurification of RNA–Protein Complexes in Escherichia coli. In: Ponchon, L. (eds) RNA Scaffolds. Methods in Molecular Biology, vol 2323. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1499-0_6
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DOI: https://doi.org/10.1007/978-1-0716-1499-0_6
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1498-3
Online ISBN: 978-1-0716-1499-0
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