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Immunophenotyping of the Medullary B Cell Compartment In Mouse Models

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Bone Marrow Environment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2308))

Abstract

B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopulation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.

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Acknowledgments

The study was supported by an ANR @RAction grant (ANR-14-ACHN-0008), an ANR JCJC grant (ANR-19-CE15-001901), a Fondation ARC grant (PJA20181208173), a grant from IdEx Université de Paris (ANR-18-IDEX-0001) to ME, and an ANR PRC grant (ANR-17-CE14-0019), an INCa grant (PRT-K 2017) and the Association Saint Louis pour la Recherche sur les Leucémies to KB.

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Authors and Affiliations

  1. Université de Paris, Institut de Recherche Saint Louis, EMiLy, INSERM U1160, Paris, France

    Amélie Bonaud

  2. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, Inserm U1160, Paris, France

    Karl Balabanian & Marion Espéli

Authors
  1. Amélie Bonaud
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  2. Karl Balabanian
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  3. Marion Espéli
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Corresponding authors

Correspondence to Amélie Bonaud or Marion Espéli .

Editor information

Editors and Affiliations

  1. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

    Marion Espéli

  2. Université de Paris, Institut de Recherche Saint Louis, OPALE Carnot Institute, EMiLy, INSERM U1160, Paris, France

    Karl Balabanian

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Bonaud, A., Balabanian, K., Espéli, M. (2021). Immunophenotyping of the Medullary B Cell Compartment In Mouse Models. In: Espéli, M., Balabanian, K. (eds) Bone Marrow Environment. Methods in Molecular Biology, vol 2308. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1425-9_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1425-9_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1424-2

  • Online ISBN: 978-1-0716-1425-9

  • eBook Packages: Springer Protocols

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